Finally, lung cancer cell lines (A549, NCI‐H1299, and CALU‐3) and human normal bronchial epithelial cell lines (BEAS‐2B) were selected to explore the aberrant expression of MIR100HG at the cellular level, and the results reflected that MIR100HG was highly expressed in human lung cancer cells (A549, NCI‐H1299, and CALU‐3) versus BEAS‐2B cells (Figure 1E), and was most significantly upregulated in A549 cells in particular; therefore, A549 cells were selected for the follow‐up study. Here, MIR100HG is linked to lung cancer.