CCK‐8, colony formation, and Transwell assays were performed to estimate lung cancer cell proliferation, migration, and invasion, and the findings reflected that downregulation of miR‐5590‐3p reversed the inhibitory effect of silencing MIR100HG on proliferation, migration, and invasion of A549 cells, and overexpression of DCBLD2 also reversed the inhibitory effect of miR‐5590‐3p upregulation on cell proliferation and metastasis (Figure 6C–F). Here, MIR100HG is linked to lung cancer.