Subsequently, through WGCNA, logistic regression, SVM, RF, and Lasso regression, we identified six Hub MRGs (NR4A1, PPP2R2A, P4HA1, TUBB6, DUSP1, NAMPT), all significantly downregulated in NAFLD samples compared to the control group. Here, NAMPT is linked to metabolic dysfunction-associated steatotic liver disease.