Subsequently, through WGCNA, logistic regression, SVM, RF, and Lasso regression, we identified six Hub MRGs (NR4A1, PPP2R2A, P4HA1, TUBB6, DUSP1, NAMPT), all significantly downregulated in NAFLD samples compared to the control group. The gene discussed is NR4A1; the disease is metabolic dysfunction-associated steatotic liver disease.