Subsequently, through WGCNA, logistic regression, SVM, RF, and Lasso regression, we identified six Hub MRGs (NR4A1, PPP2R2A, P4HA1, TUBB6, DUSP1, NAMPT), all significantly downregulated in NAFLD samples compared to the control group. This evidence concerns the gene DUSP1 and metabolic dysfunction-associated steatotic liver disease.