They also alleviated T2DM symptoms by upregulating the abundance of Gram positive bacteria (Lactobacillus, Clostridium, etc.)and downregulating the abundance of Gram negative bacteria, inhibiting the LPS lipopolysaccharide binding protein (LBP) - monocyte chemotactic protein-1 (MCP-1) - CD14-TLR4 signaling pathway. The gene discussed is TLR4; the disease is type 2 diabetes mellitus.