We immunostained and analyzed the brains of 27 individuals with known APOE genotypes (8 APOEε4/4 homozygous carriers with AD, 8 APOEε3/4 heterozygote carriers with AD, and 11 APOEε4 non-carriers (APOEε3/3) with AD (Supplementary Table 7) for FN1 and CD31 (endothelial cell marker), and COL6A2 and COL4 (a vascular basement membrane marker) (Fig. 5, Supplementary Dataset 1, Supplementary Dataset 2). This evidence concerns the gene APOE and Alzheimer disease.