Among negative-regulated genes, the analysis showed several experimentally validated target genes of miR-181 family members, such as LATS1 [25], TRIM2 [26], ACVR2A [27], NOTCH4 [28], RAP1B [29], MAP3K3 [30], PRKCD [31], ADCY1 [32], ZNF562 [33], YWHAG [34], KRAS [35], CARD11 [36], PEAK1 [37], CREB1 [38], SIRT1 [26, 39], belonging to key cancer-related signaling pathways (Fig. S2F, G; Table S4). The gene discussed is CREB1; the disease is cancer.