Notably, the CD8_Tem-GZMK subset was relatively closer to the CD8_Tex-LAYN subset compared to the CD8_Tn-LEF1 and CD8_Trm-KLRB1 subsets (Figures 5F, G), aligning with recent studies that highlight the transition from effector memory T cells to exhausted T cells during tumor progression. This evidence concerns the gene GZMK and neoplasm.