In the MVMR analysis, the results adjusted for dehydroepiandrosterone sulfate levels (DSL), estradiol levels (EL), high-density lipoprotein cholesterol (HDL-C), and body mass index (BMI) showed that increased SHBG level remains a protective factor for stroke (OR = 0.72, 95%CI = 0.59–0.87, P = 0.0011) and IS (OR = 0.70, 95%CI = 0.56–0.86, P = 0.0007), as shown in Fig. 5. Here, SHBG is linked to stroke disorder.