The results showed that the mRNA expression of most analyzed genes (ABCG8, HMGCR, DAGT, ACC1, FASN, LDLR, APOB, CPT1A, PPAR-α, SREBP1-c, and TGF-β) was not significantly different among the six groups of mice with NAFLD (Figures 6A1, A2) (p < 0.05). The gene discussed is CPT1A; the disease is metabolic dysfunction-associated steatotic liver disease.