The results showed that the mRNA expression of most analyzed genes (ABCG8, HMGCR, DAGT, ACC1, FASN, LDLR, APOB, CPT1A, PPAR-α, SREBP1-c, and TGF-β) was not significantly different among the six groups of mice with NAFLD (Figures 6A1, A2) (p < 0.05). This evidence concerns the gene TGFB1 and metabolic dysfunction-associated steatotic liver disease.