MAPT and Alzheimer disease: However, in a separate investigation, we show that early AD-related pathological changes in the Aβ42/40 biomarker were associated with quantifiable changes in brain microstructure and connectivity in Aβ-PET negative patients preceding deviations in other plasma biomarkers including t-tau, p-tau, neurofilament light chain (NfL), and glial fibrillary acidic protein (GFAP), and cortical atrophy (47).