Based on our findings, 64 cases (72.72%) were syndromic microtia [TCOF1 (43.75%), SIX2 (4.69%), and HSPA9 (4.69%)] and 24 cases (27.27%) were non-syndromic microtia [GSC exon 2 (25%), FANCB (16.67%), HOXA2 (8.33%), GSC exon 3 (8.33%), MARS1 (8.33%), CDT1 (8.33%)]. Here, TCOF1 is linked to microtia.