ATG5 and acute kidney injury: To investigate the potential role of macrophage Atg5 expression in macrophage migration to the injured kidney, we obtained BMDMs from WT or MΦ atg5−/− mice, labeled them using green CMFDA dye, injected 2 × 106 CMFDA-labeled BMDMs into WT littermate mice with induced unilateral I/R injury 16 h prior, and evaluated the number of CMFDA-labeled F4/80 double-positive cells in the kidney 3 days after AKI induction.