Polychromatic immunofluorescence revealed that SIRPB1 co-localizes with TMEM119 (microglia marker), CD86 (M1 macrophage marker), and CD163 (M2 macrophage marker) indicating that SIRPB1 is predominantly found in TAMs, which in glioma tissues exhibit both M1 and M2 characteristics (Fig. 2G). This evidence concerns the gene CD163 and glioma.