Polychromatic immunofluorescence revealed that SIRPB1 co-localizes with TMEM119 (microglia marker), CD86 (M1 macrophage marker), and CD163 (M2 macrophage marker) indicating that SIRPB1 is predominantly found in TAMs, which in glioma tissues exhibit both M1 and M2 characteristics (Fig. 2G). Here, CD86 is linked to glioma.