As illustrated in Fig. 1C, the mRNA expression of UNC5B-AS1 in the U87MG cell line was downregulated compared to that in the HEB cell line, which is consistent with our bioinformatics analysis results above and had never been reported in GBM; therefore, we focused on UNC5B-AS1 for subsequent analysis. This evidence concerns the gene UNC5B and glioblastoma.