Therefore, this study aimed to determine the expression and alterations of EPPK1 in various cancer types, evaluate its association with clinical outcomes in non-small cell lung cancer (NSCLC), and elucidate the functional role of EPPK1 and the genomic alterations that occur following EPPK1 knockout (KO) in lung cancer cells. The gene discussed is EPPK1; the disease is lung cancer.