Using R-4.1.3 software, the BLCA transcriptome data in TCGA was analyzed, revealing that the expression of SHMT1 in bladder cancer was significantly higher than that in normal tissues (P < 0.05), as shown in Fig. 1B. Further analysis using the 'paired difference analysis' method showed that the expression of SHMT1 in the tumor group was also significantly higher than that in the normal group (P < 0.05), as depicted in Fig. 1C. The analysis results of the three groups for SHMT1 showed differences, which will be discussed later. This evidence concerns the gene SHMT1 and neoplasm.