FISHtoFigure analysis reveals a statistically significant (p < 0.01, Mann–Whitney test) upregulation in expression of Cd79a and Il10 during infection (Fig. 3b).The ability to analyse and plot cellular information for specific cell types with multiplex transcriptional profiles allowed us to identify the upregulation of Cd79a+Il10+ Bregs and Cx3cr1+Il10ra+ microglia in infected specimens compared with controls, a difference which would otherwise require custom code to assess (Fig. 3c). Here, CX3CR1 is linked to infection.