We then obtained the third TF list by applying NetAct15, which identifies the enriched TFs by performing gene set enrichment analysis (GSEA, with slight adjustments15) using a curated TF-target database on the differentially expressed genes (defined as those with the adjusted p-values below 0.05 by using limma26) between the normal controls and the AML patients with IDH mutations (Fig. 2c). The gene discussed is IDH1; the disease is acute myeloid leukemia.