In this study, we aimed to evaluate the prognostic relevance of CHD4 in HNSCC patients and establish stable CHD4-knockdown HNSCC cells using the CRISPR/Cas9 gene editing system, to determine, if CHD4 could be identified as a possible target protein driving radioresistance, proliferation and colony formation ability in irradiated HNSCC cells. This evidence concerns the gene CHD4 and head and neck squamous cell carcinoma.