We therefore performed Visium sequencing on six melanoma metastases (three CTLA4res, one PD1res, one anti-CTLA4 resistant mucosal and one ICB naïve), and defined melanoma cell states as characterized by differential expression of MITF, MKI67, NGFR, AXL and TAP1. Consensus clustering of 2,766 tumor cell-enriched spots resulted in five groups where four were characterized mainly by differential expression of MITF and TAP1 (Fig. 3A). This evidence concerns the gene AXL and neoplasm.