The present study analyzed the staining intensity and immunolocalization of TLR1-10 and NF-κB in the different epithelial layers, basement membrane (BM) zone, endothelium, and infiltrative zone of oral squamous cell carcinoma (OSCC) tissue sections and results were compared with the staining intensity in healthy oral mucosa from control patients. The gene discussed is NFKB1; the disease is oral cavity squamous cell carcinoma.