We first examined the protein expression levels of TOP2A in five MM cell lines (NCI-H929, MM.1S, RPMI-8226, U266, and OPM2) and found that NCI-H929 and RPMI-8226 showed higher levels of TOP2A while TOP2A expression in MM.1S and U266 was relatively low (Fig 2f). Here, TOP2A is linked to Miyoshi myopathy.