Through different time points analysis, we found that MSA-2 significantly stimulated TNF-α, IL-10, IL-6 and INF-β expression in both plasma (Fig. 4A–D) and tumor (Fig. 4E–H) and this expression reached the highest level around 4–6 days after MSA-2 injection and down to normal spectrum after 16 days. The gene discussed is TNF; the disease is neoplasm.