In the multivariable model that adjusted sex, age, weight, blood pressure, HBA1C, T2DM duration, calcium, phosphorus, parathyroid hormone, lipids and renal function, vitamin D deficiency maintained its significant association with subclinical DPN (OR 1.276, 95% CI 1.086-1.501, P = 0.003). This evidence concerns the gene PTH and vitamin D deficiency.