In addition, we obtained the same results in the in vivo experiments, regardless of whether it was rat serum or colon tissue homogenate, TNF-α and IL-6 in the AP + EsA group were both lower than those in the AP model group and the difference was statistically significant (p < 0.001), while the difference between the AP + EsA group and the control group was not statistically significant, Moreover, there was no significant difference between the EsA group and the control group (Figure 1(d)−1(g)). This evidence concerns the gene IL6 and alkaline phosphatase measurement.