In our previous single-cell analysis of the TME in CRC, we found that in the CD44-enriched region of the TME of the colon, there was an increase in crosstalk between SPP1+TAM and Foxp3+regulatory T cells (Tregs), which may increase the immunosuppressive microenvironment of CRC (54). This evidence concerns the gene SPP1 and colorectal carcinoma.