According to our results, CD44 showed positive relation to following factors: main immune checkpoints of testicular germ cell tumor (TGCT), ACC, KICH, LGG, LIHC, LUAD, and OV; TMB values of COAD, LGG, and UCEC; and MSI values of COAD, READ, and UCEC. The gene discussed is CD44; the disease is testicular germ cell tumor.