In another study, a neoantigenic peptide (Adpgk) from MC-38 colon cancer was also supplemented with ten consecutive positively charged lysines (10 K-Adpgk) in order to obtain a cationic peptide, which self-assembled with the TLR-9 agonist, CpG oligodeoxynucleotide (CpG ODN) adjuvant, and directly formed antigen/adjuvant-integrated nano-partitioned complexes (PCNPs) via electrostatic interactions for potent tumour immunotherapy (43). This evidence concerns the gene ADPGK and malignant colon neoplasm.