In terms of the risks for early spontaneous abortion, there was an interaction between higher plasma HCY in early pregnancy, serum folate deficiency in early pregnancy and maternal MTHFR 677TT genotype, and there was an interaction between higher plasma HCY and serum folate deficiency in early pregnancy, and there was an interaction between serum folate deficiency in early pregnancy and maternal MTHFR 677TT genotype. This evidence concerns the gene MTHFR and folate deficiency.