The potential PCa sEV protein markers including RTN4, LARS1, LAMB1, LAMB2, PXDN, NRP1, ABI3BP, FLNC, and SPON2 are highly expressed in sEVs from mPCa cell lines, but absent or minimally expressed in sEVs from a localised primary PCa cell line or a normal prostate epithelial cell line; URP2, DNAJA1, ADK, Histone H4, CLTC, AHCY, MYH9, Histone H3.1, RAN, FLNA, and CD151 are highly expressed in sEVs from PCa cell lines, but absent or minimally expressed in sEVs from normal prostate epithelial cells. Here, RAN is linked to posterior cortical atrophy.