Because the expression of HSPA4 was significantly correlated with the tumor proliferation signature in TCGA GC dataset (Fig. 2A), we established stable HSPA4-overexpressing GC cells (MKN45-HSPA4 and HGC27-HSPA4) and HSPA4 knockdown cells (AGS-sh) to explore the function of HSPA4 in GC cells (Fig. 2B). This evidence concerns the gene HSPA4 and gastric cancer.