The induction of autoantigen by miR-574-5p was further confirmed by the demonstration that in vivo induction of miR-574-5p transgenic expression induced a significant increase in serum autoantigen Sp100 (supplemental Figure S7g), whereas miR-574-5p knockdown in the lupus-prone mice resulted in a significant reduction of both Trim21/Ro52 and Sp100 (Fig. 9e-f). This evidence concerns the gene TRIM21 and systemic lupus erythematosus.