The results of qRT-PCR analysis of relative mRNA displayed in the Fig. 6B further supported the upregulation of TNF-α (10.69-fold), p53 (4.89-fold), BAX (6.23-fold), and downregulation of Bcl2 (0.041-fold) in PCOS rats (Fig. 6B). This evidence concerns the gene TP53 and polycystic ovary syndrome.