Likewise, searching the ENCODE database, we found that the PRNP promoter is bound by the TCF7L2 gene-encoded TCF4 transcription factor, the main β-catenin co-factor in colon and liver, as well as H3K27ac (H3 histone acetylated on Lysine 27) and H3K4me3, which both mark active chromatin, in HCT116 colon cancer cells (Fig. 1E). Here, TCF4 is linked to malignant colon neoplasm.