Next, we evaluated the relationship between TUBA1B expression and matrix and immune scores across various cancers using the ESTIMATE algorithm, and the results revealed that TUBA1B expression was positively correlated with Stromal Score, Immune Score, and ESTIMATE Score in GBMLGG, KIRC, KIRP, LUAD, PAAD, PRAD, and THCA. This evidence concerns the gene TUBA1B and cancer.