Specifically, TUBA1B exhibited the highest expression in the basal subtype of BRCA, HM-SNV subtype of COAD, ESCC subtype of ESCA, Basal subtype of HNSC, C2c-CIMP subtype of KIRP, Classic-like subtype of LGG, Primitive subtype of LUSC, Proliferative subtype of OV, Kinase signaling subtype of PCPG, 6-FOXA1 subtype of PRAD, HM-indel subtype of STAD, and MSI subtype of UCEC. This evidence concerns the gene FOXA1 and esophageal squamous cell carcinoma.