In the mPFC, however, the local over-expression of LCN2 (Fig. 2b–d) effectively induced anxiety-like behaviors in naïve, unstressed mice (Fig. 2e–g) but did not alter the general locomotor behaviors (Fig. S3q–s), highlighting the anxiogenic role of LCN2 preferentially in the mPFC. This evidence concerns the gene LCN2 and Anxiety.