In contrast, immune signaling pathways were suppressed in ICI-resistant SILlow tumors, which also lacked the expression of IFNG, IFNLR1, TNF and TNFR genes, limiting the pro-inflammatory immune stimulating activities within the TME, which is corroborated by our findings of poor lymphocyte infiltration and checkpoint expression in the SILlow melanomas. The gene discussed is TNF; the disease is melanoma.