Using a Mitochondria/Cytosol Protein Isolation Kit, followed by western blot analysis, GLUD1 and AKT proteins were detected in both the mitochondria and cytosol of HCC cells (Figure 3J), suggesting that GLUD1 interacts with AKT and affects AKT activation directly in these regions of tumor cells. The gene discussed is AKT1; the disease is neoplasm.