As shown in Figure 1D–F, the gene expression levels of GLUD1 were lower in HCC patients aged < 60 years (compared to ≥60 years), with the alpha-fetoprotein (AFP) level ≤20 ng/ml (compared to >20 ng/ml), and with a fibrosis Ishak score ≤2 (compared to >2). Here, AFP is linked to hepatocellular carcinoma.