Among the candidate genes downstream of GLUD1 in HepG2 cells identified by RNA sequencing analysis (Figure 2A), we determined that GLUD1 inhibited the expression level of IL-32, a cytokine that plays a role in HCC development (Pan et al., 2011; Kang et al., 2012). The gene discussed is GLUD1; the disease is hepatocellular carcinoma.