Second, we also identified that proximal tubular Sirt1 was downregulated by SGLT2/GLUT2-mediated intracellular signals in DN, which is in accordance with the finding that NAD and sirtuins are more influenced by high glucose-induced stimulation than hypertension-induced glomerulosclerotic injuries [19]. The gene discussed is SIRT1; the disease is liver dysplastic nodule.