FHIT gene methylation was conducted by MS-PCR (n = 66) while as analysis of FHIT mRNA expression levels were conducted in corresponding 40 cases of ALL (25 males and 15 females; mean age, of 12.2 ± 3.4 years; with an age range, of 1–27 years) and 40 healthy (age and sex matched) children 30 males and 12 females; mean age, of 12.3 ± 4.1 years; with an age range, of 1–30 years). Here, FHIT is linked to acute lymphoblastic leukemia.