PROM1 and breast cancer: Furthermore, we found that CD133-high BC significantly enriched signaling pathways that are activated in CSCs: WNT/β catenin signaling (normalized enrichment score (NES) = 1.77, FDR = 0.21), Hedgehog signaling (NES = 1.27, FDR = 0.24), and NOTCH signaling (NES = 1.63, FDR = 0.12) in TCGA, which was validated in the METABRIC cohort: WNT/β-catenin signaling (NES = 1.62, FDR = 0.03), Hedgehog signaling (NES = 1.28, FDR = 0.16), and NOTCH signaling (NES = 1.39, FDR = 0.10) (Fig. 2B).