We identified a TUBB6 p.(Glu410Lys) variant in syndromic familial ptosis pedigree ENG_CML; this substitution has been reported as P/LP for tubulinopathies in four paralogs (TUBB2A, TUBB2B, TUBB3, and TUBB4A; ClinVar Variation IDs: 986830, 1195195, 6967, 135658). Here, TUBB2B is linked to ptosis.