To confirm the role of SIRT5 in AML cells, the SIRT5 expression in AML cells was knocked down, and results showed that mRNA expressions of SIRT5 in HL-60 and KG1a cell lines after sh-SIRT5 transfection were significantly reduced (Figure 2a), in addition to considerably decreased protein levels (Figure 2b). The gene discussed is SIRT5; the disease is acute myeloid leukemia.