Moreover, the binding of AGO2 to the miRNA/ssDNA hybrids protected their degradation from RNase H. Accordingly, AGO2 could act as a guardian to make sure the normal procedure of NamiRNA‐mediated transcriptional activation in nucleus by stabilizing the miRNA/ssDNA hybrids during osteosarcoma metastasis. The gene discussed is AGO2; the disease is osteosarcoma.