We next analyzed the correlation between the percentage of 21 immune cells and Klotho gene expression in GSE104954 (CKD) and GSE108112 (CKD) and found that the percentage of Neutrophils and T cells gamma delta was negatively correlated with KL expression in both GSE104954 (CKD) and GSE108112 (CKD) datasets, and T cells regulatory (Tregs) was positively correlated with KL expression in both datasets (all P < 0.05) (Fig. 3D). This evidence concerns the gene KL and chronic kidney disease.