Considering that Klotho could modulate monocyte inflammatory factor release [56], and there was also a negative correlation between the percentage of monocytes in GSE104954 (CKD) and GSE108112 (CKD) and the expression of KL (with P < 0.05 in GSE108112) (Fig. 3D), we chose monocytes for the transwell invasion assay. This evidence concerns the gene KL and chronic kidney disease.