In the GSE104954 (CKD) and GSE108112 (CKD) datasets, the most relevant biological process with Klotho gene is fatty acid β-oxidation, followed by fatty acid metabolic process and lipid metabolic process (Fig. 4A, E), and the cellular components related to the Klotho gene are mitochondrion, mitochondrial matrix and extracellular exosome (Fig. 4B, F), and the molecular functions associated with Klotho gene are catalytic activity, oxidoreductase activity, flavin adenine dinucleotide binding, and electron carrier activity (Fig. 4C, G). The gene discussed is KL; the disease is chronic kidney disease.