KL and chronic kidney disease: We also analyzed two independent tubulointerstitial transcriptome datasets for CKD (data from the Nephroseq v5 database) and found that KL expression was positively correlated with eGFR in both the Sampson Nephrotic Syndrome TubInt and Woroniecka Diabetes TubInt datasets (R = 0.4844 and 0.6475, P = 0.0004 and 0.0011) (Fig. 1N, M), which further confirmed that Klotho gene expression in the tubulointerstitium was well correlated with renal function.