GSDMD and liver dysplastic nodule: Conversely, the silencing of caspase-4 or GSDMD via siRNA significantly reduces pyroptosis-associated modifications in vitro.478 Compared to WT mice, GSDMD−/− mice showed reduced pyroptosis and improved kidney injury-related indices.478,479 In addition, glomerular endothelial cell (GECs) injury emerges as a pivotal pathological process during the early stages of DN.