The E3 ubiquitin ligase CDC20 reduces tumor cell pyroptosis through ubiquitinated degradation of GSMDE.305 The Dub USP48 and OTUD4 promote GSDME-mediated pyroptosis by deubiquitinating and stabilizing GSDME, which increases the sensitivity of tumor cells to treatment.306,307 It is clear that we are just beginning to understand the regulatory role of ubiquitination on GSDMs, and the mechanisms of GSDM recognition and ubiquitination remain to be elucidated. Here, OTUD4 is linked to neoplasm.