In the subset of patients with PAD, pedal MAC remained significantly associated with an increased the risk of amputation in both crude model (OR 3.03, 95% CI 1.77–5.20, p < .001) and after adjusting for sex, age, albumin, hemoglobin, and diabetic retinopathy (OR 2.14, 95% CI 1.14–4.02, p = .018). The gene discussed is ALB; the disease is diabetic retinopathy.