To test if SR-B1 and S1PR1 form complexes in cells within atherosclerotic plaques, we generated S1pr1eGFP/eGFP/ApoEKO/KO mice and fed them a high fat, high cholesterol, atherogenic diet for 8 weeks to promote atherosclerosis development in their aortic sinuses. This evidence concerns the gene S1PR1 and atherosclerosis.