To verify that the TFEB clusters observed were not the result of non-specific antibody binding in fixed tissue specimens and could be also observed in vitro, we differentiated human embryonic stem cells (hESC)-derived neurons from a healthy control case (wt) and an isogenically derived hESC line lacking GBA (GBA KO) to model for the reduced GCase activity observed in GBA-PD/PDD cases. Here, TFEB is linked to Parkinson disease.