Comparison of tumor type-specific MIB-1 LI values showed a significant difference, as Pilocytic astrocytomas °1 were characterized by the highest mean MIB-1 LI values, followed by pediatric-type diffuse low-grade gliomas (including diffuse astrocytomas °2, MYB or MYBL1-altered; and diffuse low-grade gliomas °2, MAPK-pathway altered) and Gangliogliomas °1 (2.9 vs 2.6 vs 2.1%, p = 0.04, Kruskal − Wallis test). Here, MIB1 is linked to diffuse astrocytoma.