Apoptosis induced by PSMD1 depletion, as shown in Fig. 2, was further confirmed in additional HCC cells with different p53 statuses, as evidenced by increased annexin V/PI co-staining (Fig. 4c, supplementary Fig. 2b) and PARP protein cleavage (Fig. 4d), whereas normal cells did not exhibit apoptosis. Here, ANXA5 is linked to hepatocellular carcinoma.