To test the optimized TELL-Seq protocol with the first type of targets, we focused on seven genomic regions associated with hereditary predisposition to cancer: BRCA1 (target length: 198.4 kb), BRCA2 (target length: 187.5 kb), APC (target length: 199.6 kb), MSH2 (target length: 201.1 kb), MSH6 (target length: 197.5 kb), MLH1 (target length: 201.7 kb), and PMS2 (target length: 197.5 kb). The gene discussed is MLH1; the disease is cancer.